Protein Resins
Bioworld Protein-A Gold™ (1.5- 5 nm), 0.5 mL
Protein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules, including colloidial gold, without affecting the antibody binding site on the molecule. Protein A is immobilized on the nanogold beads.Specifications:Particle size 1.5-2.5 nm (monodisperse), nominal 15 nm (colloidal gold) Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 20% glycerol, and 15 mM sodium azide. Storage: 2-8 C
Bioworld MagneZoom™ Protein-G Kit, 1 mL
MagneZoom Protein G (superparamagnetic beads) of average 5 μm in diameter, covalently coated with recombinant Protein G. Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.Kit Components:• Protein-G MagneZoom™ (25mg/ml), 1ml• Binding/Wash Buffer • Elution Buffer • Neutralization Buffer• 1×SDS Sample Buffer This kit requires MagneZoom paramagnetic bead station (20160000-1) separating beads.
Bioworld Protein-A Gold™ (1.5- 5 nm), 1 mL
Protein-A Gold Protein-A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. It is capable of binding to the Fc portion of immunoglobulins, especially IgG from a large number of species. Protein-A may be conjugated with various reporter molecules, including colloidial gold, without affecting the antibody binding site on the molecule. Protein A is immobilized on the nanogold beads.Specifications:Particle size 1.5-2.5 nm (monodisperse), nominal 15 nm (colloidal gold) Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, 20% glycerol, and 15 mM sodium azide. Storage: 2-8 C
Bioworld VIEWIP PROTEIN-A RED SEPARO
VIEWIP PROTEIN-A RED SEPAROPORE (AGAROSE) 4B-CL: viewIP Protein-A Red Separopore 4B-CL Red-Colored Affinity matrixhas immobilized Recombinant Protein-A. Convenient for quantitative capturing of antigen/antibody complexes in immunoprecip...
Bioworld PROTEIN-A SEPAROPORE 4B PR
PROTEIN-A SEPAROPORE 4B, PREPACKED COLUMN 2X 2 ML: Protein A Separopore 4B Affinity matrix with immobilized Protein-A (from staphylococcus aureus) by CNBr activation coupling method. For purification and/or fractionation of IgG subclasse...
Bioworld Protein G-Coated Microplates, 5 Plate(s)
ProteinG coatedmicroplates provide alternative methods for immobilizing antibodies for ELISA and other plate-based assay techniques. These plates are uniformly and stably coated with Protein G. They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.Specifications:Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption Orient antibodies for maximum antigen-binding capacity Immobilize antibodies without prior purification Ensure minimal variation (<5% well-to-well) from consistent coating Reduce nonspecific binding because plates are pre-blockedBinds up to 2.5µg antibody/well (100µL coating volume)
Sigma Aldrich Fine Chemicals Biosciences Protein G SepharoseR fast
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex affinity chromatography on Sepharose-coupled human IgG and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10 with the strongest binding at pH 4 and 5 and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.P3296-5Ml updated product number is GE17-0618-01
AdipoGen Lipocalin-2 rat rec
Protein. Rat lipocalin-2 aa 1-198 is fused at the C-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in PBS. Purity >90% SDS-PAGE. Lipocalin-2 is an iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development.
Bioworld MagneZoom™ A Protein-A Magnetic Beads, 5 mL
Recombinant protein-A covalently linked to MagneZoom (magnetic beads). For magnetic separation and isolation of IgG subclasses from ascites, serum, or cell culture supernatants.Technical Specifications:Particle size: 2-5µmLigand: rProtein A Binding Capacity: 0.20mg rabbit IgG/ml beadspH stability: 4-9Supplied as 25mg/ml suspensionRequires MagneZoom paramagnetic bead station (20160000-1) for sepaating beads.Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY.
Bioworld MagneZoom™ COOH, 10 mL
Carboxy-terminated Magnetic BeadsSilica-coated uniform super paramagnetic beads with surface carboxyl group. Proteins and nucleic acids can be covalently bound to these beads with the retention of biological activity.Specifications:Size: 5-10µm.COOH terminatedBinding: up to 20mg/ml.pH stability: 4-9Supplied as 25mg/ml suspension.Requires MagneZoom paramagnetic bead station (20160000-1) for bead separation.Usage : bioWORLD's products are supplied for LABORATORY RESEARCH USE ONLY.
CUBE BIOTECH PureCube Fe-NTA MagBeads; 25 ml
25 ml of Fe-NTA Magnetic Beads for Phosphopeptide Enrichment, 25% slurry
AdipoGen CD137 mouseFc h rec
Protein. The extracellular domain of mouse CD137 aa 1-185 is fused at the C-terminus to the Fc portion of human IgG1. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm filtered solution in PBS. Purity >95% SDS-PAGE. CD137 4-1BB is a member of the tumor necrosis factor TNF receptor family. CD137 is expressed by activated T cells, dendritic cells, NK cells, granulocytes and cells of blood vessel walls at sites of inflammation. CD137 has costimulatory activity for activated T cells. Crosslinking of CD137 enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. CD137 was shown to enhance immune activity to eliminate tumors in mice.
AdipoGen GITRL Soluble mouse rec
Protein. The extracellular domain of mouse GITRL aa 45-173 is fused at the N-terminus to a FLAG -tag. Source HEK 293 cells. Endotoxin content <0.1EU/µg purified protein LAL test Lonza. Liquid. 0.2µm-filtered solution in 10mM glycine, pH 10.0. Binds to mouse GITR. Purity >90% SDS-PAGE. GITRL Glucocorticoid-induced TNF receptor ligand is expressed on dendritic cells DC, monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues e.g. testis. GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis.
American Research Products Inc Mouse Clara cell protein CC16
Mouse Clara cell protein CC16 ELISA Kit from CUSABIO Detection Range 0 156 ng/mL-10 ng/mL Sensitivity 0 039 ng/mL Sample serum plasma tissue homogenates Method Sandwich
Genscript Corporation Protein A Resin FF
GenScript s Protein A Resin FF is an affinity chromatography media designed for easy one-step purification of classes subclasses and fragments of immunoglobulins from biological fluids and from cell culture media The recombinant protein A ligand is coupled to 4% highly cross-linked agarose The coupling is optimized to have a high binding capacity for immunoglobulins The static binding capacity of Protein A Resin FF is greater than 40 mg rabbit IgG/ ml settled resin The dynamic binding capacity will vary depending on several factors such as target antibody flow rate etc Table 1 lists the characteristics of Protein A Resin FF br br Protein A a bacterial cell wall protein isolated from i Staphylococcus aureus /i binds to mammalian IgGs mainly at their Fc region Native protein A has five IgG binding domains and many other domains with unknown functions Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce n